test kit Group A Rotavirus Antigen Rapid Test Kit (Card, Cassette)

Rotavirus (RV) belongs to Rotavirus genus in family Reoviridae and recognized as the major etiology agents of acute gastroenteri

 

Group A Rotavirus Antigen Rapid Test Kit (Colloidal Gold )

CatalogNo.         NAME                PACKAGE SIZE:

BG301C     Rotavirus Antigen Rapid Card      20 tests/Kit

SUMMARY

Rotavirus (RV) belongs to Rotavirus genus in family Reoviridae and recognized as the major etiology agents of acute gastroenteritis in infants and young children worldwide.  It was said that about 600~800 thousand people died each year from rotaviruses.

Even in the developed countries in which the mortality is much lower then developing countries, rotavirus is responsible for 30~60% of all cases of acute gastroenteritis reported each year.

The highest prevalence of the diarrhea in infants in China is experienced all year round except summer. It is approved that 40~60% of the cases is caused by rotavirus.

Enteral Rotavirus infections are transmitted via the fecal-oral route from person to person or contaminated objects can be the source of infection. The golden standard for the diagnosis of Rotavirus infections is direct virus detection by electron microscopy.

PRINCIPLE

The kit uses a sandwich solid phase immunochromatographic assay to detect group A rotavirus antigen in human stool specimen. The rotavirus antibody and gold labeled conjugate are coupled to the surface of nitrocellulose membrane. Add an aliquot of diluted stool sample to the sample well of the test cassette. If the sample contains rotavirus antigens, a antigen-antibody-gold complexes will form. These complexes move on the nitrocellulose membrane by capillary action toward the test line region on which rotavirus specific antibodies are immobilized. As the complexes reach the test line, they will bind to the antibody on the membrane in the form of a pink line. A second red control line will always appear in the result window to indicate that the test has been correctly performed and the test device functions properly. If rotavirus antigen is not present or lower than the detection limit of the test, only the control line will be visible.If the control line dose not developed, the test is invalid.The test results are intended to aid in the diagnosis of rotavirus infection and to monitor the effectiveness of therapeutic treatment.

 

CHARACTERISTIC

 

1. A sandwich solid phase immunochromatographic assay which can catch any subgroup antigen of Group A Rotavirus.

2. High sensitivity and perfect specificity

3. Clear background and easy to read

4. Fast and easy to operate , no instrument and  technique needed

5. Room temperature is enough to store.

 

PROCEDURE

1. Bring all materials and specimens to room temperature (8~30°C).

2. Add 500μl sample diluent into the test tube.

3. Add proper specimens (S:5 mm in diameter, L: 50μl) into the tube to make the concentration of the solution up to 5%~10% and mix thoroughly.

4. Deliver 100μl (2~3drops ) of diluted stool sample to the sample well of the test card.

5. Read the result in 5~10 minutes. A strong positive sample may show result earlier.

Note: Results after 15 minutes may not be accurate.

                           

test kit Group A Rotavirus Antigen Rapid Test Kit (Card, Cassette)